The mechanism of action of cobamide coenzyme in the ribonucleotide reductase reaction.

Investigation of solvent effect on the active site energy of Carbonic Anhydrase and Ribonucleotide Reductase

Enzymes catalyze many biological reactions. The rates of chemical reaction in the presence ofenzymes are, in some cases, accelerated more than 10 orders of magnitude relative to thecorresponding rates in solution.In this paper a comparison between optimized structures of two enzyme molecules in aspect ofenergy and dipole moment in different conditions including presence of metallic ion, without...

Hydrogen donor specificity of cobamide-dependent ribonucleotide reductase and allosteric regulation of substrate specificity.

Studies on the mechanism of adenosylcobalamin-dependent ribonucleotide reduction by the use of analogs of the coenzyme.

A series of 17 analogs of 5'-deoxy-5'-adenosylcobalamin(adenosylcobalamin) have been synthesized with modifications in the base or ribose moiety of the nucleoside ligand. These analogs have been examined for their effects on reactions catalyzed by the ribonucleotide reductase of Lactobacillus leichmannii. All the analogs are inhibitors of ATP reduction in the presence of adenosylcobalamin as co...

Studies of the Mechanism of Action of Cobamide Coenzymes.

Aerobacter aerogenes converts propanediol to propionaldehyde, and ethylene glycol to acetaldehyde.' Dioldehydrase, the enzyme that catalyzes this reaction, has been purified 200-fold and a requirement for any one of three cobamide coenzymes and for a monovalent metal ion has been established.2 The present work seeks to extend the limited information3 now available on the mechanism of action of ...

Hypolipidemic effects of total flavonoide extracted from the leaves of Actinidia kolomikta in rats fed a high-fat diet

Objective(s): This study was to investigate the antihyperlipidemic and antioxidant effect of total flavonoid extract from Actinidia kolomikta (TFAK) in hyperlipidemia induced by a high-fat diet. Materials and Methods: Male SD rats were randomly divided into 6 groups: normal group, model (hyperlipidemic diet) group, hyperlipedemic diet supplemented with TFAK (50, 100 and 200 mg/kg) and simvastat...